		Technical Data
R1075-01B	RANK (Tumor Necrosis Factor Receptor Superfamily Member 11A, Osteoclast Differentiation Factor Receptor, ODFR, Receptor Activator of NF-KB, CD265, TNFRSF11A)

Description:
Receptor activator of NF-kB (RANK) is a recently cloned member of the TNFR superfamily with no significant homology to other members of this family. RANK ligand (RANKL/TRANCE/ OPGL) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival. The cytoplasmic domain of RANK interacts with TRAF2, TRAF5 and TRAF6. Overexpression of RANK activates NF-kB and c-Jun-terminal kinase (JNK) pathway. Recent studies have shown that RANK interaction with TRAF6 activates NF-kB, whereas JNK activation is mediated through binding of RANK to TRAF2.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
RAW cell lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to aa603-613 of human RANK. (Genbank accession number: NP_003830.)

Purity:

Purified by Protein G affinity Chromatography.

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

Specificity:

Recognizes human RANK. Species Crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Anderson,D.M., Maraskovsky,E., Billingsley,W.L., Dougall,W.C., et al. A homologue of the TNF receptor and its ligand enhance T-cell growth and dendritic-cell function. Nature 390 (6656), 175-179 (1997). 2. Darnay,B.G., Ni,J., Moore,P.A. and Aggarwal,B.B. Activation of NF-kappaB by RANK requires tumor necrosis factor receptor-associated factor (TRAF) 6 and NF-kappaB-inducing kinase. Identification of a novel TRAF6 interaction motif. J. Biol. Chem. 274 (12), 7724-7731 (1999). 3. Hsu,H., Lacey,D.L., Dunstan,C.R., Solovyev,I., Colombero,A., et al. Tumor necrosis factor receptor family member RANK mediates osteoclast differentiation and activation induced by osteoprotegerin ligand. Proc. Natl. Acad. Sci. U.S.A. 96 (7), 3540-3545 (1999). 4. Dougall,W.C., Glaccum,M., Charrier,K., Rohrbach,K., Brasel,K., De Smedt,T., et al. RANK is essential for osteoclast and lymph node development. Genes Dev. 13 (18), 2412-2424 (1999). 5. Karsdal,M.A., Hjorth,P., Henriksen,K., Kirkegaard,T., Nielsen,K.L., et al. Transforming growth factor-beta controls human osteoclastogenesis through the p38 MAPK and regulation of RANK expression. J. Biol. Chem. 278 (45), 44975-44987 (2003).