Technical Data
R1075-04D
RANK (Receptor Activator of NF-kB, Nuclear Factor kappa B, Receptor Activator of NFkB, Activator of NFkB, CD265, EOF, FEO, mRANK, NFKB Activator, Osteoclast Differentiation Factor Receptor, ODFR, OFE, PDB2, Tumor Necrosis Factor Receptor Superfamily Membe
Description:
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. Receptor activator of NF-kB (RANK) is a recently cloned member of the TNFR superfamily with no significant homology to other members of this family. RANK ligand (RANKL/TRANCE/ OPGL) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival. The cytoplasmic domain of RANK interacts with TRAF2, TRAF5 and TRAF6. Overexpression of RANK activates NF-kB and c-Jun-terminal kinase (JNK) pathways. Recent studies have shown that RANK interaction with TRAF6 activates NF-kB, whereas JNK activation is mediated through binding of RANK to TRAF2.

Applications:
Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Paraffin-embedded sections
Western Blot: 1-2ug/ml
Flow Cytometry: Cell surface
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k9C30Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
A fusion protein containing aa326-616 of human RANK.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity:
Recognizes human RANK. In RAW cells, a band at ~97kD. Recognizes an epitope located between aa330-427 of human RANK. Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Anderson, D.M, Maraskovsky, E., Billingsley, W.L., Dougall, W.C., Tometsko, M.E., Roux, E.R., Teepe, M.C., DuBose, R.F., Cosman, D., and Gaibert, L. Nature 390: 175-179 (1997). 2. Wong, B.R., Josien, R., Lee, S.W., Sauter, B., Li, H-L., Steinman, R.M., and Choi, Y. J. Exp. Med. 186: 2075-2080. 3. Darnay, B.G., Haridas, V., Ni, J., Moore, P.A., and Aggarwal, B.B. J. Biol. Chem 273: 20551-20555 (1998). 4. Darnay, B.G., Ni, J., Moore, P.A., and Aggarwal, B.B. J. Biol. Chem. 274: 7724-7731 (1999).