		Technical Data
R1075-04F	RANK (Tumor Necrosis Factor Receptor Superfamily Member 11A, Osteoclast Differentiation Factor Receptor, ODFR, Receptor Activator of NF-KB, CD265, TNFRSF11A) (PE)

Description:
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. Receptor activator of NF-kB (RANK) is a recently cloned member of the TNFR superfamily with no significant homology to other members of this family (1). RANK ligand (RANKL/TRANCE/ OPGL) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival (2). The cytoplasmic domain of RANK interacts with TRAF2, TRAF5 and TRAF6 (3,4). Overexpression of RANK activates NF-kB and c-Jun-terminal kinase (JNK) pathways (3). Recent studies have shown that RANK interaction with TRAF6 activates NF-kB, whereas JNK activation is mediated through binding of RANK to TRAF2 (4).

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry (Cell Surface): 2-5ug/10e6 cells
Optimal dilutions to be determined by the researcher.

Positive Control:
Activated T cells or RAW cells

Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

Type	Isotype	Clone	Grade
Mab	IgG1,l	11E333	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		4°C Do Not Freeze	Blue Ice	Human	Mouse

Concentration:

~0.5mg/ml

Immunogen:

Recombinant corresponding to protein containing extracellular domain of human RANK.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, 0.05% sodium azide. Labeled with R-Phycoerythrin (PE).

Specificity:

Recognizes human RANK. Species Crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Anderson, D.M, Maraskovsky, E., Billingsley, W.L., Dougall, W.C., Tometsko, M.E., Roux, E.R., Teepe, M.C., DuBose, R.F., Cosman, D., and Gaibert, L. Nature 390: 175-179 (1997). 2. Wong, B.R., Josien, R., Lee, S.W., Sauter, B., Li, H-L., Steinman, R.M., and Choi, Y. J. Exp. Med. 186: 2075-2080. 3. Darnay, B.G., Haridas, V., Ni, J., Moore, P.A., and Aggarwal, B.B. J. Biol. Chem 273: 20551-20555 (1998). 4. Darnay, B.G., Ni, J., Moore, P.A., and Aggarwal, B.B. J. Biol. Chem. 274: 7724-7731 (1999).