Technical Data
R1075-04F
RANK (Tumor Necrosis Factor Receptor Superfamily Member 11A, Osteoclast Differentiation Factor Receptor, ODFR, Receptor Activator of NF-KB, CD265, TNFRSF11A) (PE)
Description:
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. Receptor activator of NF-kB (RANK) is a recently cloned member of the TNFR superfamily with no significant homology to other members of this family (1). RANK ligand (RANKL/TRANCE/ OPGL) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival (2). The cytoplasmic domain of RANK interacts with TRAF2, TRAF5 and TRAF6 (3,4). Overexpression of RANK activates NF-kB and c-Jun-terminal kinase (JNK) pathways (3). Recent studies have shown that RANK interaction with TRAF6 activates NF-kB, whereas JNK activation is mediated through binding of RANK to TRAF2 (4).

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry (Cell Surface): 2-5ug/10e6 cells
Optimal dilutions to be determined by the researcher.

Positive Control:
Activated T cells or RAW cells

Storage and Stability:
May be stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG1,l11E333Affinity Purified
SizeStorageShippingSourceHost
100ug4C Do Not FreezeBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Recombinant corresponding to protein containing extracellular domain of human RANK.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% sodium azide. Labeled with R-Phycoerythrin (PE).
Specificity:
Recognizes human RANK. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Anderson, D.M, Maraskovsky, E., Billingsley, W.L., Dougall, W.C., Tometsko, M.E., Roux, E.R., Teepe, M.C., DuBose, R.F., Cosman, D., and Gaibert, L. Nature 390: 175-179 (1997). 2. Wong, B.R., Josien, R., Lee, S.W., Sauter, B., Li, H-L., Steinman, R.M., and Choi, Y. J. Exp. Med. 186: 2075-2080. 3. Darnay, B.G., Haridas, V., Ni, J., Moore, P.A., and Aggarwal, B.B. J. Biol. Chem 273: 20551-20555 (1998). 4. Darnay, B.G., Ni, J., Moore, P.A., and Aggarwal, B.B. J. Biol. Chem. 274: 7724-7731 (1999).