Technical Data
R1075-10S
RANKL (Receptor Activator of Nuclear Factor kappa B Ligand, Receptor Activator of NFkB Ligand, CD254, hRANKL2, hRANKL-2, Osteoclast Differentiation Factor, ODF, Osteoprotegerin Ligand, OPGL, sOdf, SOFA, TNF-related Activation-induced Cytokine, TRANCE, Tum
Description:
RANKL is a recently identified member of tumor necrosis factor family. TRANCE (TNF-related activation-induced cytokine receptor) or RANKL {receptor activator of NF-kB (RANK) ligand} has been implicated in interactions between T cells and dendritic cells. RANK ligand binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival. RANKL is also important in T- and B-cell maturation. As OPGL/ODF (Osteoprotegerin Ligand/Osteoclast differentiation factor), the same protein can both activate mature osteoclasts and mediate osteoclastogenesis. OPGL/TRANCE deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceMouseRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa161-177 of mouse Trance (AQPFAHLTINAASIPSG), GenBank no. gi|12643362|sp|O35235|TNF11_MOUSE.
Purity:
Purified by Protein G affinity Chromatography.
Form
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity:
Recognizes mouse RANKL. Species sequence homology: human and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hsu, H., Lacey, D.L., Dunstan, C.R., Solovyev, I., et al. Proc. Natl. Acad. Sci. USA 96: 3540-3545 (1999). 2. Lacey, D.L. Timms, E,. Tan, H.L., Kelly, M.J. , et al. Cell 93: 165-176 (1998). 3. Kong, Y-Y., Yoshida, H., Sarosi, I., Tan, H-L., et al. Nature 397: 315-323 (1999). 4. Wong, B.R., Josien, R., Lee, S.W., Sauter, B., Li, H-L., Steinman, R.M., and Choi, Y. J. Exp. Med. 186: 2075-2080. 5. Anderson, D.M, Maraskovsky, E., Billingsley, W.L., Dougall, W.C., Tometsko, M.E., Roux, E.R., Teepe, M.C., DuBose, R.F., Cosman, D., and Gaibert, L. Nature 390: 175-179 (1997).