RANK is a new member of the tumor necrosis factor receptor superfamily as an osteoblast-expressed ligand that stimulates osteoclastic differentiation, and a T-cell-expressed ligand that enhances dendritic cells survival.
Suitable for use in Western Blot and Immunohistochemistry. Not suitable for Flow Cytometry. Other applications not tested.
Western Blot: 1-2ug/ml Detects a band of ~35-40kD
Immunohistochemistry (Paraffin): 5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|Recombinant mouse RANKL (aa 1-317).|
|Purified by Protein G affinity chromatography.|
|Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.|
|Recognizes mouse RANKL. Species Crossreactivity: human|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Multiple myeloma disrupts the TRANCE/ osteoprotegerin cytokine axis to trigger bone destruction and promote tumor progression: R.N. Pearse, et al.; PNAS 98, 11581 (2001) 2. Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines: P. Fiumara, et al.; Blood 98, 2784 (2001)3. Gene expression of osteoclast differentiation factor is induced by lipopolysaccharide in mouse osteoblasts via Toll-like receptors: T. Kikuchi, et al.; J. Immunol. 166, 3574 (2001) 4. Gamma-glutamyltranspeptidase stimulates receptor activator of nuclear factor-kappaB ligand expression independent of its enzymatic activity and serves as a pathological bone-resorbing factor: S. Niida, et al.; J. Biol. Chem. 279, 5752 (2004)|