Technical Data
R1075-16
RANKL (OPGL, ODF, Trance, Receptor Activator of NFkB Ligand)
Description:
RANK is a new member of the tumor necrosis factor receptor superfamily as an osteoblast-expressed ligand that stimulates osteoclastic differentiation, and a T-cell-expressed ligand that enhances dendritic cells survival.

Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Not recommended for use in Flow cytometry or Immunoprecipitation. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:1000
Immunohistochemistry: paraffin sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG16D725Highly Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanRat
Concentration:
~1mg/ml
Immunogen:
Recombinant human soluble RANKL (aa 151-316).
Purity:
Purified from concentrated hybridoma tissue culture supernatant. 95% (SDS-PAGE).
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes human RANKL.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
The molecular triad OPG/RANK/RANKL: involvement in the orchestration of pathophysiological bone remodeling: S. Theoleyre, et al.; Cytokine Growth Factor Rev. 15, 457 (2004)