Technical Data
R1075-77
Rank Ligand, Recombinant Human
2ug
10ug
100ug
10ug
100ug
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Technical Data |
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R1075-77 |
Rank Ligand, Recombinant Human |
2ug 10ug 100ug |
| Growth Factors, Cytokines | Storage: -70°CShipping: Blue Ice |
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Recombinant Human soluble RANKL produced in E. coli is a single, non-glycosylated polypeptide chain containing 176 amino acids and having a molecular mass of 20006 Dalton. Endotoxin: <0.01ng/ug or <0.1EU/ug as per LAL Biological Activity:RANK ligand is fully biologically active when compared to standard. The activity is determined by its ability to induce osteoclast formation in RAW264.7 cells using a conc. of 5.0-10.0 ng/ml and by dose dependent stimulation of IL-8 production in human PBMC which was found to be <10ng/ml. AA Sequence: MEKAMVDGSW LDLAKRSKLE AQPFAHLTIN ATDIPSGSHK VSLSSWYHDR GWAKISNMTF SNGKLIVNQD GFYYLYANIC FRHHETSGDL ATEYLQLMVY VTKTSIKIPS SHTLMKGGST KYWSGNSEFH FYSINVGGFF KLRSGEEISI EVSNPSLLDP DQDATYFGAF KVRDID Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: 20kD |
Source: Recombinant, human, E. coli Purity: 98% as determined by RP-HPLC; Reducing and non-reducing SDS-PAGE. Form: Supplied as a lyophilized powder. Reconstitute with sterile dH2O PBS to 0.1-1mg/ml, which can be further diluted into other aqueous solutions. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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