Technical Data
R1075-77
Rank Ligand, Recombinant Human
2ug
10ug
100ug
Molecular Biology Storage: -70CShipping: Blue Ice
Recombinant Human soluble RANKL produced in E. coli is a single, non-glycosylated polypeptide chain containing 176 amino acids and having a molecular mass of 20006 Dalton.

Endotoxin: <0.01ng/ug or <0.1EU/ug as per LAL

Biological Activity:RANK ligand is fully biologically active when compared to standard. The activity is determined by its ability to induce osteoclast formation in RAW264.7 cells using a conc. of 5.0-10.0 ng/ml and by dose dependent stimulation of IL-8 production in human PBMC which was found to be <10ng/ml.

AA Sequence:
MEKAMVDGSW LDLAKRSKLE AQPFAHLTIN ATDIPSGSHK VSLSSWYHDR GWAKISNMTF SNGKLIVNQD GFYYLYANIC FRHHETSGDL ATEYLQLMVY VTKTSIKIPS SHTLMKGGST KYWSGNSEFH FYSINVGGFF KLRSGEEISI EVSNPSLLDP DQDATYFGAF KVRDID

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O, aliquot and store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
20kD
Source: Recombinant, human, E. coli
Purity: 98% as determined by RP-HPLC; Reducing and non-reducing SDS-PAGE.
Form: Supplied as a lyophilized powder. Reconstitute with sterile dH2O PBS to 0.1-1mg/ml, which can be further diluted into other aqueous solutions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.