Technical Data
R1076-03
sRANKL, Recombinant, Human (soluble RANK Ligand, Receptor Activator of NFkB Ligand)
2ug
10ug
Growth Factors, Cytokines Storage: -20CShipping: RT
RANKL binds to tnfrsf11b/opg and to tnfrsf11a/rank. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive t-cell proliferation. May be an important regulator of interactions between t-cells and dendritic cells and may play a role in the regulation of the t-cell-dependent immune response. sRANKL may also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.

Recombinant Human soluble RANKL is a single, non-glycosylated polypeptide chain containing 176 amino acids and having a molecular mass of 20006 Dalton.

Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Lys-Ala-Met.

Biological Activity:
RANK ligand is fully biologically active when compared to standard. The activity is determined by its ability to induce osteoclast formation in RAW264.7 cells using a concentration of 5-10ng/ml and by dose dependent stimulation of IL-8 production in human PBMC.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O, 0.1% BSA or HSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
20kD
Source: E. coli
Purity: ~ 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Concentration: ~0.1mg/ml
Form: Supplied as a lyophilized powder from 10mM Tris, pH 7.5. Reconstitute with sterile ddH2O, 0.1% BSA or HSA to 0.1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.