Technical Data
R1076-04
sRANKL, Recombinant, Mouse (soluble RANK Ligand, Receptor Activator of NFkB Ligand)
2ug
10ug
Molecular Biology Storage: -20CShipping: Blue Ice
RANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANK (receptor) was originally identified as a dendritic-cell-membrane protein, which by interacting with RANKL augments the ability of dendritic cells to stimulate nave T-cell proliferation in a mixed lymphocyte reaction, to promote the survival of RANK + T cells, and to regulate T-cell-dependent immune response. RANKL, which is expressed in a variety of cells including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage. Recombinant murine sRANKL is a 19.4kD polypeptide comprising the TNF homologous region of RANKL (174 amino acid residues).

Biological Activity:
Measured by its ability to induce osteoclast formation on murine RAW264.7 cells using a concentration of 5-10ng/ml

AA Sequence:
PAMMEGSWLD VAQRGKPEAQ PFAHLTINAA SIPSGSHKVT LSSWYHDRGW AKISNMTLSN GKLRVNQDGF YYLYANICFR HHETSGSVPT DYLQLMVYVV KTSIKIPSSH NLMKGGSTKN WSGNSEFHFY SINVGGFFKL RAGEEISIQV SNPSLLDPDQ DATYFGAFKV QDID

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile ddH2O, aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Country of Origin:
USA

Molecular Weight:
19.4kD
Source: Murine, recombinant protein expressed in E. coli
Purity: Highly purified ( 98% by SDS-PAGE and HPLC).
Endotoxin: 0.1ng/ug (1EU/ug).
Form: Supplied as a lyophilized powder in 5mM Tris, pH 7.6, 75mM sodium chloride.
Reconstitution: Reconstitute with sterile dH2O, to a concentration of 0.1-1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Ohishi, M., et al., Journal of Immunology 174(5): 3024-3031 (2005). 2. Wang, K., et al., Journal of Biological Chemistry 279(51): 53,848-53,856 (2004). 3. Okamatsu, Y., et al., Journal of Immunology 173(3): 2084-2090 (2004). 4. Hirotani, H., et al., Journal of Biological Chemistry 279(14): 13,984-13,992 (2004). 5. Fakruddin, J.M. & Laurence, J., J. Biol. Chem. 278(48): 48,251-48,258 (2003).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.