Technical Data
R1076-05G
sRANKL (soluble RANK Ligand, Receptor Activator of NFkB Ligand)
Description:
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA (Sandwich, Indirect): 0.5-2.0ug/ml using 100ul/well antibody solution. In conjunction with compatible secondary reagents, allows the detection 0.2-0.4ng/well of recombinant hsRANKL.
Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANKL is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute by adding sterile 40-50% glycerol, ddH2O. Aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4C (-20C Glycerol)Blue IceHumanGoat
Concentration:
~1mg/ml
Immunogen:
Highly pure (> 98%) recombinant human soluble RANKL
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.4. Reconstitute with sterile 40-50% glycerol, dH2O to 0.1-1mg/ml.
Specificity:
Recognizes human soluble RANKL.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.