		Technical Data
R1076-05G	sRANKL (soluble RANK Ligand, Receptor Activator of NFkB Ligand)

Description:
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA (Sandwich, Indirect): 0.5-2.0ug/ml using 100ul/well antibody solution. In conjunction with compatible secondary reagents, allows the detection 0.2-0.4ng/well of recombinant hsRANKL.
Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANKL is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		4°C (-20°C Glycerol)	Blue Ice	Human	Goat

Concentration:

~1mg/ml

Immunogen:

Highly pure (> 98%) recombinant human soluble RANKL

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a lyophilized powder from PBS, pH 7.4. Reconstitute with sterile 40-50% glycerol, dH2O to 0.1-1mg/ml.

Specificity:

Recognizes human soluble RANKL.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.