Technical Data
R1076-05J
sRANKL (soluble RANK Ligand, Receptor Activator of NFkB Ligand)
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Technical Data |
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R1076-05J |
sRANKL (soluble RANK Ligand, Receptor Activator of NFkB Ligand) |
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Description: Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: To detect msRANKL by direct ELISA (using 100ul/well antibody solution) a concentration of at least 0.5ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4ng/well of recombinant msRANKL. Western Blot: To detect msRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant msRANKL is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Reconstitution: Reconstitute to 0.2mg/ml with sterile dH2O or sterile 40-50% glycerol for long term storage. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Country of Origin: USA |
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