RANTES, Recombinant, Human (Regulated on Activation, Normal T-cell Expressed and Secreted)
|Molecular Biology||Storage: -20°CShipping: RT|
RANTES is a CC-chemokine that can signal through the CCR1, CCR3, CCR5 and US28 (cytomegalovirus receptor) receptors. It is a chemoattractant towards monocytes, memory T cells (CD4+/CD45RO), basophils, and eosinophils. RANTES also has the capability to inhibit certain strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
Recombinant human RANTES is a 7.8kD protein containing 68 amino acid residues including the four highly conserved cysteine residues present in the CC-chemokines.
Amino Acid Sequence:
SPYSSDTTPC CFAYIARPLP RAHIKEYFYT SGKCSNPAVV FVTRKNRQVC ANPEKKWVRE YINSLEMS
Determined by its ability to chemoattract human blood monocytes using a concentration range of 1-10ng/ml.
Responding Cells (partial list):
Concentration Range for most in vitro applications:
Lyophilized RANTES is soluble in water and most aqueous buffers. Reconstitute in sterile dH2O to a concentration of 0.1-1mg/ml. It is recommended that further dilutions be made into buffer containing carrier protein or medium containing serum.
Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile dH2O. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Country of Origin:
Source: Human, recombinant expressed in E. coli
Purity: 98% by SDS-PAGE and HPLC analyses.
Form: Supplied as a lyophilized powder without additives.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.