Technical Data
R1100-05
RANTES, Recombinant, Human (Regulated on Activation, Normal T-cell Expressed and Secreted)
5ug
20ug
Molecular Biology Storage: -20CShipping: RT
RANTES is a CC-chemokine that can signal through the CCR1, CCR3, CCR5 and US28 (cytomegalovirus receptor) receptors. It is a chemoattractant towards monocytes, memory T cells (CD4+/CD45RO), basophils, and eosinophils. RANTES also has the capability to inhibit certain strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).

Recombinant human RANTES is a 7.8kD protein containing 68 amino acid residues including the four highly conserved cysteine residues present in the CC-chemokines.

Amino Acid Sequence:
SPYSSDTTPC CFAYIARPLP RAHIKEYFYT SGKCSNPAVV FVTRKNRQVC ANPEKKWVRE YINSLEMS

Endotoxin:
0.1ng/ug (1EU/ug)

Biological Activity:
Determined by its ability to chemoattract human blood monocytes using a concentration range of 1-10ng/ml.

Responding Cells (partial list):
Monocytes

Concentration Range for most in vitro applications:
0.2-20ng/ml

Reconstitution:
Lyophilized RANTES is soluble in water and most aqueous buffers. Reconstitute in sterile dH2O to a concentration of 0.1-1mg/ml. It is recommended that further dilutions be made into buffer containing carrier protein or medium containing serum.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute by adding sterile dH2O. Aliquot and store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Country of Origin:
USA

Molecular Weight:
7.8kD
Source: Human, recombinant expressed in E. coli
Purity: 98% by SDS-PAGE and HPLC analyses.
Form: Supplied as a lyophilized powder without additives.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.