Technical Data
RANTES, Recombinant, Human (Regulated on Activation, Normal T-cell Expressed and Secreted)
Molecular Biology Storage: -20CShipping: Blue Ice
Recombinant Human Rantes produced in E.Coli is a single, non-glycosylated polypeptide chain containing 68 amino acids and having a molecular mass of 7809.2 Dalton. Recombinant Human Rantes is purified by proprietary chromatographic techniques.

Amino Acid Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Tyr-Ser-Ser.

Biological Activity:
ED50 Range = 5.0-2.0ng/ml, determined by the dose dependent chemotaxis of THP-1 cells.

Storage and Stability:
Lyophilized powder may be stored at -20C. Reconstitute to nominal volume by adding sterile ddH2O, carrier protein. Aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E. coli
Purity: 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Concentration: ~1mg/ml
Form: Supplied as a lyophilized powder. Reconstitute with sterile ddH2O, carrier protein to 100ug/ml, which can then be further diluted to other aqueous solutions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Role of platelet-derived chemokines (RANTES and ENA-78) after stem cell transplantation. Transpl Immunol 2006 Apr;15(4):247-53 2. Early prediction of sepsis-induced disseminated intravascular coagulation with interleukin-10, interleukin-6, and RANTES in preterm infants. Clin Chem 2006 Jun;52(6):1181-9 3. Rat peritoneal mast cells release regulated upon activation normal T cell expressed and secreted (RANTES) after TNF-alpha activation. J Biol Regul Homeost Agents 2005 Jul-Dec;19(3-4):130-5 4. Preclinical evaluation of synthetic -2 RANTES as a candidate vaginal microbicide to target CCR5. Antimicrob Agents Chemother 2006 Apr;50(4):1497-509 5. Mast cell beta-tryptase selectively cleaves eotaxin and RANTES and abrogates their eosinophil chemotactic activities. J Immunol 2006 Mar 15;176(6):3788-95 6. Serum levels of IL-6 and its soluble receptor, TNF-alpha and chemokine RANTES in scorpion envenomed children: their relation to scorpion envenomation outcome. Toxicon 2006 Mar 15;47(4):437-44

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.