Technical Data
RANTES, Recombinant, Mouse (Regulated on Activation, Normal T-cell Expressed and Secreted, CCL5)
Molecular Biology Storage: -20CShipping: Blue Ice
Recombinant corresponding to mouse RANTES expressed in E. coli.

Molecular Weight:

Endotoxin: ~0.1ng/ug

Biological Activity:
ED50 range=5.0-20ng/ml, determined by the dose dependent chemotaxis of THP-1 cells. The optimal concentration for each specific application should be determined by an initial dose-response assay.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Purity: ~95% (SDS-PAGE)
Concentration: Not determined
Form: Supplied as a lyophilized powder. BSA free. No preservative added. Reconstitute with sterile ddH2O to 0.1-1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Schall, T.J., N.J. Simpson, and J.Y. Mak (1992) Molecular cloning and expression of the murine RANTES cytokine: Structural and functional conservation between mouse and man. Eur. J. Immunol. 22:1477-1481. 2. Haelens, A., A. Wuyts, P. Proost, S. Struyf, G. Opdenakker, and J. van Damme (1996) Leukocyte migration and activation by murine chemokines. Immunobiology 195:499-521. 3. Fischer, F.R., Y. Luo, M. Luo, L. Santambrogio, and M. Dorf (2001) RANTES-induced chemokine cascade in dendritic cells. J. Immunol. 167:1637-1643. 4. Zhang, Y., Q. Zhai, Y. Luo, and M. Dorf (2002) RANTES-mediated chemokine transcription in astrocytes involves activation and translocation of p90 ribosomal S6 protein kinase (RSK). J. Biol. Chem. 277:19042-19048. 5. Luo, Y., M. Berman, Q. Zhai, F. Fisher, S.R. Abromson-Leeman, Y. Zhang, W.A. Kuziel, C. Gerard, and M.E. Dorf (2002) RANTES stimulates inflammatory cascades and receptor modulation in murine astrocytes. Glial 39:19-30.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.