Technical Data
RANTES, Recombinant, Rat (Regulated on Activation, Normal T-cell Expressed and Secreted)
Molecular Biology Storage: -70CShipping: Blue Ice
Recombinant Rat Rantes produced in E. coli is a single, non-glycosylated polypeptide chain containing 68 amino acids and having a molecular mass of 7876 Dalton.

Biological Activity: Recombinant Rantes is fully biologically active when compared to standard. The activity is determined by the ability to chemoattract total human lymphocytes and murine T-cells at a concentration between 1-10ng/ml.

AA Sequence:

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O, aliquot and store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Purity: ~95% Analysis by RP-HPLC; Reducing and non-reducing SDS-PAGE.
Form: Supplied as a lyophilized powder. Reconstitute with sterile dH2O PBS to 0.1-1mg/ml, which can be further diluted into other aqueous solutions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Rat basophilic leukemia cells (RBL-2H3) generate prostaglandin D2 (PGD2) after regulated upon activation, normal T-cell expressed and secreted (RANTES) activation. Int J Biol Markers 2006 Oct-Dec;21(4):211-7
2. Umbilical serum concentrations of chemokines (RANTES and MGSA/GRO-alpha) in preterm and term neonates. Pediatr Int 2006 Dec;48(6):586-90
3. Expression and secretion of RANTES (CCL5) in granulomatous calcified tissue before and after lipopolysaccharide treatment in vivo. Calcif Tissue Int 2007 Jan;80(1):60-7
4. Platelet-derived chemokine RANTES may be a sign of restenosis after percutaneous coronary intervention in patients with stable angina pectoris. Platelets 2006 Dec;17(8):565-70
5. Upregulating promoter polymorphisms of RANTES relate to atopic dermatitis. Int J Immunogenet 2006 Dec;33(6):423-8
6. Simvastatin down regulates mRNA expression of RANTES and CCR5 in posttransplant renal recipients with hyperlipidemia. Transplant Proc 2006 Nov;38(9):2899-904

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.