Technical Data
Rap1 (hRap1)
Human Rap1 was the first identified ortholog of the yeast telomeric protein scRap1, which shares 3 conserved sequence motifs in common. HRap1 is located at telomeres and affects telomere length by being recruited to telomeres by TRF2 instead of directly binding to the telomere like scRap1. The hRap1 cDNA codes for a protein of 399 amino acids with approximate molecular weight of 47kD. The hRap1 protein does not interact with TRF1. TRF2 is a ubiquitously expressed protein that is implicated in the control of telomere length(1). TRF2, like TRF1 contains a Myb-related DNA binding motif. It binds to duplex TTAGGG repeats and is localized to all human telomeres in metaphase chromosomes(1). TRF2 is thought to protect chromosome ends by maintaining the correct structure at telomere termini(2). The use of mutant forms of TRF2 has implicated a role for TRF2 in the prevention of senescence in primary human cells(2). Recently, it has been shown that inhibition of TRF2 resulted in apoptosis in a subset of mammalian cell types(3).

Suitable for use in Western Blot, Immunocytochemistry.
Recommended Dilution:
Western Blot: 2ug/ml
Immunocytochemistry: 2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ug-20CBlue IceHumanRabbit
Synthetic peptides corresponding amino acids 212-235 of human Rap1.
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Recognizes human Rap1 (hRap1)
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Broccoli, D, et al., Nature Genetics 17:231-239 (1997). 2. Van Steensel, B., et al., Cell 92:401- 413 (1998). 3. Karlseder, J., et al., Science 283: 1321-1325 (1999). 4. Li B, et al., Cell 26; 101(5):471-483 (2000). 5. Scherthan H, et al., Mol Biol Cell 11(12):4189-203 (2000).