Technical Data
R1120-14
RIAM
Description:
RIAM (a Rap1-GTP-interacting adapter molecule) defines a family of adaptor molecules that contain a RA-like (Ras association) domain, a PH (pleckstrin homology) domain, and various proline-rich motifs (1). RIAM directly binds to Ena/VASP proteins, important modulators of cell migration that localize to focal adhesions and stress fibers, and co-localizes with them at focal adhesions in response to Ras activation. Moreover, RIAM directly binds to activated Ras in a phosphoinositide-dependent manner. RIAM is believed to be the first direct link between Ras signalling and cytoskeletal remodelling via Ena/VASP proteins during cell migration and spreading (2).

Applications:
Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:50
Western Blot: 1:1000-1:2000
Immunohistochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG9E519Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to residues near the N-terminus of human RIAM.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% Glycerol.
Specificity:
Recognizes human RIAM at ~110kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lafuente EM, et al. Dev Cell 7(4):585-95, 2004. 2. Jenzora A et al. FEBS Lett 579(2):455-63, 2005