Technical Data
R1198-01M
NRAS (Neuroblastoma RAS Viral (v-ras) Oncogene Homolog, N-Ras, ALPS4, AV095280, GTPase NRas, HRAS1, N-Ras Protein Part 4, NRAS1, OTTHUMP00000013879, OTTMUSP00000023521, Transforming Protein N-Ras, v-Ras Neuroblastoma RAS Viral Oncogene Homolog)
Description:
The Ras protein is a subfamily of small GTPases that are involved in signal transduction. They are activated by ligand-binding receptor tyrosine kinases such as EGFR, PDGFR, CSF and FGF. These kinases transiently convert RAS-GDP (inactive form) to RAS-GTP, the active form of RAS. The main genes are HRAS, NRAS and KRAS.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot:1:500-1:5000
Optimal dilutions to be determined by the researcher.

Hybridoma:
FO myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,k12L6Affinity Purified
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant corresponding to aa1-186 from human RAS expressed in E.coli.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid In PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes human RAS.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Rocks O, et al. (2006). Current Opinion in Cell Biology 18 (4): 351-7. 2. Wennerberg K, et al. (March 2005). J. Cell. Sci. 118 (Pt 5): 843-6. 3. Rotblat B, et al. (2008). Methods Enzymol 439: 467-89.