		Technical Data
R1198-01M	NRAS (Neuroblastoma RAS Viral (v-ras) Oncogene Homolog, N-Ras, ALPS4, AV095280, GTPase NRas, HRAS1, N-Ras Protein Part 4, NRAS1, OTTHUMP00000013879, OTTMUSP00000023521, Transforming Protein N-Ras, v-Ras Neuroblastoma RAS Viral Oncogene Homolog)

Description:
The Ras protein is a subfamily of small GTPases that are involved in signal transduction. They are activated by ligand-binding receptor tyrosine kinases such as EGFR, PDGFR, CSF and FGF. These kinases transiently convert RAS-GDP (inactive form) to RAS-GTP, the active form of RAS. The main genes are HRAS, NRAS and KRAS.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot:1:500-1:5000
Optimal dilutions to be determined by the researcher.

Hybridoma:
FO myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2a,k	12L6	Affinity Purified

Size		Storage	Shipping	Source	Host
50ul		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Recombinant corresponding to aa1-186 from human RAS expressed in E.coli.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid In PBS, pH 7.4, 0.1% sodium azide.

Specificity:

Recognizes human RAS.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Rocks O, et al. (2006). Current Opinion in Cell Biology 18 (4): 351-7. 2. Wennerberg K, et al. (March 2005). J. Cell. Sci. 118 (Pt 5): 843-6. 3. Rotblat B, et al. (2008). Methods Enzymol 439: 467-89.