Technical Data
RasGRP3, phosphorylated (pT133)(RASGRP3, KIAA0846)
The RasGRPs are a family of Ras activators that possess diacylglycerol-binding C1 domains (1). RasGRP3 is an activator for H-Ras, R-Ras and Ras-associated protein-1/2, and has emerged as an important mediator of signaling downstream from receptor coupled phosphoinositide turnover in B and T cells (2). It has been demonstrated that RasGRP3 undergoes phosphorylation at Thr-133 upon B cell receptor cross-linking, thereby resulting in its activation. Moreover, mutation of Thr-133 in RasGRP3 alone severely impaired its ability to activate Ras in B cell receptor signaling (3).

Suitable for use in Western Blot and Immunocytochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:1000-1:10,000
Immunocytochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
100ul-20°CBlue IceHumanRabbit
Not determined
A phospho-specific peptide corresponding to residues surrounding Threonine 133 of human RasGRP3.
Supplied as a liquid in PBS, pH 7.2, 0.05% BSA, 0.01% sodium azide, 50% glycerol.
Recognizes human RasGRP3 phosphorylated at Threonine 133. Species crossreactivity: Mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Coughlin JJ, et al. J Immunol 175(11):7179-84, 2005 2. Yang D, et al. Oncogene May 23, 2011 3. Aiba Y, et al. Proc Natl Acad Sci U S A 101(47):16612-7, 2004