Technical Data
R1200-12U
RASIP1 (Ras-interacting Protein 1, Rain)
Description:
Required for the proper formation of vascular structures that develop via both vasculogenesis and angiogenesis. Acts as a critical and vascular-specific regulator of GTPase signaling, cell architecture, and adhesion, which is essential for endothelial cell morphogenesis and blood vessel tubulogenesis. Regulates the activity of Rho GTPases in part by recruiting ARHGAP29 and suppressing RhoA signaling and dampening ROCK and MYH9 activities in endothelial cells. May act as effector for Golgi-bound HRAS and other Ras-like proteins. May promote HRAS-mediated transformation.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:8000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to CANYRHGPPVATSP, from RASIP1, at C-terminal (NP_060275.2).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:
Recognizes RASIP1. Species sequence homology: Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Natalia Y. Mitin, Melissa B. Ramocki, Alfred J. Zullo, Channing J. Der, Stephen F. Konieczny, and Elizabeth J. Taparowsky Identification and Characterization of Rain, a Novel Ras-interacting Protein with a Unique Subcellular Localization J. Biol. Chem. 2004;279: 22353-22361.