Technical Data
R1310-01
Red Fluorescent Protein (RFP)
Description:
Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene.

Applications:
Suitable for use in Western Blot. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:10,0001:30,000 when detected with an anti-chicken horseradish peroxidase-conjugated secondary by ECL or by a colorimetric assay using DAB.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1906: IgY, Chicken Pab Rb x Ch
I1906-10: IgY, Chicken (HRP) Rb x Ch
I1906-12: IgY, Chicken (FITC) Rb x Ch
I1906-14: IgY, Chicken (AP) Rb x Ch

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgYAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceChicken
Concentration:
~1mg/ml
Immunogen:
Recombinant red fluorescent protein (RFP), highly purified by affinity chromatography on Nickel column.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in 10mM Tris, pH 7.5, 100mM sodium chloride, 0.02% sodium azide.
Specificity:
Recognizes red fluorescent protein (RFP). Shows no reactivity to GFP.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.