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Technical Data |
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R1310-01B |
Red Fluorescent Protein (dsRed) |
100ug |
| BioSeparation? | Storage: -20°CShipping: Blue Ice |
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The recombinant RFP (dsRed) was expressed and purified from transformed E. coli using a method that ensures high purity and maximal RFP fluorescence. Endotoxin was removed, so the protein is suitable for in vivo injection or cell culture applications. The protein is a 27.6kDa monomers containing a N-terminus 6x His-Tag with Ex./Em. = 557/585nm, extinction coefficient (e 280nm = 34380 M-1 Cm-1). Endotoxin: <0.1ng/ug Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: 27.6 kDa |
Source: E. coli Purity: ~97% (SDS-PAGE, HPLC) Form: Supplied as a lyophilized powder. Reconstitute with sterile ddH2O to a concentration of 1mg/ml. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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