Technical Data
R1362-01
Reelin (RELN, RL, Reeler)
Description:
Reelin is a 400kD, extracellular matrix glycoprotein secreted by several neurons. It is a serine protease that degrades fibronectin and laminin. Reelin also binds lipoprotein receptor superfamily members APOER2 and VLDLR which transduce signals important for neuronal positioning during brain development and synaptic plasticity in the adult brain. In vivo, Reelin undergoes proteolytic processing at two sites, generating three Reelin fragments. The central fragment is sufficient for binding and signaling via the lipoprotein receptors. Three isoforms of mouse Reelin that differ in their C-terminal fragments have been described. Within its central regions, mouse reelin shares 95% and 98% amino acid sequence identity with human and rat reelin, respectively

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:250-1:1000
Immunoprecipitation: 1:10-1:500
Immunohistochemistry (Paraffin): 1:250-1:500
Immunohistochemistry: 1:10-1:2000
Immunofluorescence (IC): 1:500
Optimal dilutions to be determined by the researcher.

Positive Control:
IHC labeling of Cajal-Retzius cells in the embryonic mouse telencephalon.

Storage and Stability:
May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG19C51Affinity Purified
SizeStorageShippingSourceHost
50ug4C Do Not FreezeBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to aa164-189 of Reelin (NM_005045.2). Cellular Localization: Secreted
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% sodium azide.
Specificity:
Recognizes Reelin. Expected to react with a wide range of species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tissir F & Goffinet AM. Nat Rev Neurosci 4:496-505 (2003). 2. de Bergeyck V et al. J Neurosci Methods 82:17-24 (1998).