Technical Data
R1362-01
Reelin (RELN, RL, Reeler)
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Technical Data |
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R1362-01 |
Reelin (RELN, RL, Reeler) |
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Description: Reelin is a 400kD, extracellular matrix glycoprotein secreted by several neurons. It is a serine protease that degrades fibronectin and laminin. Reelin also binds lipoprotein receptor superfamily members APOER2 and VLDLR which transduce signals important for neuronal positioning during brain development and synaptic plasticity in the adult brain. In vivo, Reelin undergoes proteolytic processing at two sites, generating three Reelin fragments. The central fragment is sufficient for binding and signaling via the lipoprotein receptors. Three isoforms of mouse Reelin that differ in their C-terminal fragments have been described. Within its central regions, mouse reelin shares 95% and 98% amino acid sequence identity with human and rat reelin, respectively Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry (paraffin-embedded sections). Other applications not tested. Recommended Dilution: Western Blot: 1:1000; detects a band of ~400-450, 300 and 180-200kD. Immunohistochemistry: Paraffin-embedded sections Optimal dilutions to be determined by the researcher. Positive Control: IHC labeling of Cajal-Retzius cells in the embryonic mouse telencephalon. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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