Technical Data
R1460-40
Renin-1 (Angiotensinogenase, Kidney Renin)
Description:
Mouse Renin is a secreted, 42-47 kD glycosylated member of the peptidase A1 family. It is an aspartyl protease that cleaves angiotensinogen to form angiotensin I. In mouse, there are two genes that code for Renin. One is in the submandibular gland and the other is in the kidney. The two mature Renin molecules are 95% amino acid (aa) identical. Renal Renin (Renin-1) is synthesized as a 381 aa proform (aa 22-402). In the kidney, pro-Renin is proteolytically cleaved after Thr71 to generate a mature enzyme. Mouse pro-Renin shares 70% and 85% aa sequence identity with human and rat pro-Renin, respectively.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and ELISA. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1-0.2ug/ml
ELISA: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseGoat
Concentration:
As reported
Immunogen:
NS0-derived recombinant mouse Renin-1 (aa22-402) Accession # P06281
Purity:
Purified by Immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile ddH2O.
Specificity:
Recognizes mouse Renin-1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.