Technical Data
R1585-02M
Resistin (RETN, RETN1, RSTN, Adipose Tissue-Specific Secretory Factor, ADSF, FIZZ3, Cysteine-rich Secreted Protein A12-alpha-like 2, Cysteine-Rich Secreted Protein FIZZ3, C/EBP-epsilon-Regulated Myeloid-Specific Secreted Cysteine-Rich Protein, HXCP1, RSTN
Description:
RELM:FIZZ family of proteins are Cysteine-rich proteins consist of a highly conserved C-terminal cysteine rich domain and play a vital role in regulation of adipogenesis and insulin resistance. Resistin, a human homolog of mouse resistin, is a novel member of this family. It is a 12kD adipokine which is produced by both brown and white adipose tissue and is mainly secreted by adipocytes. Its levels are down-regulated by insulin and have been shown to be elevated during Type 2 diabetes, indicating a critical role in regulating adipogenesis and insulin resistance. It promotes aortic smooth muscle cell proliferation through activation of ERK1:2 and PI3:Akt signaling pathways. It is expressed in macrophages and functions as a pro-inflammatory cytokine by up-regulating IL-6 and activating NF-kappaB signaling pathway. Its expression is regulated by numerous factors which include PPAR-alpha, Insulin, Growth and gonadal hormones, TNF-alpha, Endothelin-1 and certain pro-inflammatory cytokines like IL-6.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to aa79-90 [GSWDVRAETTCH] of human Resistin.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes human Resistin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Steppan et al. (2001) Nature 409: 307-312 2. Banerjee RR et.al J. Biol Chem., 276, 25970-25973 (2001) 3. HB Hartman et.al J. Biol. Chem., 277 (22); 19754-19761 (May 31, 2002) 4. Patel L et.al Biochem Biophys Res Commun. 300(2):472-6 (2003 Jan 10) 5. Bokarewa M et.al J Immunol. 174(9):5789-95 (2005 May 1)