Resistin, Recombinant, Mouse (ADSF, Adipose Tissue-Specific Secretory Factor, FIZZ3, Found in Inflammatory Zone, Adipose-specific cysteine-rich secreted protein A12-alpha)
|Growth Factors, Cytokines||Storage: -20°CShipping: Blue Ice|
Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteine-rich secreted proteins (monomeric peptide contains 11 cysteine residues) referred to as the RELM family, and is also described as ADSF (Adipose Tissue-Specific Secretory Factor) or FIZZ3 (Found in Inflammatory Zone 3). Mouse resistin is expressed as a 114 amino acid prepeptide; its hydrofobic Nterminal 20 amino acid signal peptide is cleaved before its secretion. Mouse resistin circulates in blood as a homodimeric protein consisting of two 94 amino acid polypeptides, which are disulfide-linked via Cys26.
Resistin may be an important link between obesity and insulin resistance. Mouse resistin, specifically produced and secreted by adipocyte, acts on skeletal muscle myocytes, hepatocytes and adipocytes themselves so that it reduces their sensitivity to insulin. Steppan et al. have suggested that resistin suppressed the ability of insulin to stimulate glucose uptake. They have also suggested that resistin was present at elevated levels in blood of obese mice, and was down regulated by fasting and by antidiabetic drugs. Way et al., on the other hand, have found that resistin expression is severely suppressed in obesity and is stimulated by several antidiabetic drugs. Other studies have shown that mouse resistin increases during the differentiation of adipocytes, but it also seems to inhibit adipogenesis. In contrast, the human adipogenic differentiation is likely to be associated with a down regulation of resistin gene expression.
A DNA sequence encoding the mature mouse Resistin protein sequence Ser 21-Ser 114 (Steppan, C.M. et al., 2001, Nature 409:307-312) was expressed in E. coli.
Recombinant mouse Resistin preparation contains a mixture of monomeric and disulfide-linked homodimeric proteins. Each monomer contains 94 amino acid residues and has a calculated molecular mass of approximately 10kD.
Measured by its ability to inhibit mouse 3T3-L1 cell adipocyte differentiation (Kim, K.H. et al., 2001, J. Biol. Chem. 276:11252-11256). The ED50 for this effect is typically 5-25ug/ml.
Endotoxin: 0.1ng/ug (1EU/ug).
It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 10ug/ml.
Storage and Stability:
Lyophilized samples are stable for up to 12 months from date of receipt at -20°C to -70°C. Upon reconstitution, this cytokine can be stored under sterile conditions at 2-8° C for one month or at -20 to -70° C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Source: Murine, recombinant expressed in E. coli
Purity: 95% by SDS-PAGE analysis.
Form: Supplied as a lyophilized powder in sterile-filtered PBS, pH 8.0, BSA.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Resistin regulates human choriocarcinoma cell invasive behaviour and endothelial cell angiogenic processes. J Endocrinol 2006 Jun;189(3):691- 699 2. Expression and regulation of resistin in osteoblasts and osteoclasts indicate a role in bone metabolism. J Cell Biochem 2006 May 23; 3. Development of recombinant-based mass spectrometric immunoassay with application to resistin expression profiling. Anal Chem 2006 May 15;78(10):3271-6 4. Increased serum resistin in elite endurance athletes with high insulin sensitivity. Diabetologia 2006 May 10; 5. Increase in adiponectin levels during pioglitazone therapy in relation to glucose control, insulin resistance as well as ghrelin and resistin levels. J Endocrinol Invest 2006 Mar;29(3):231-6 6. Adiponectin and resistin in PCOS: a clinical, biochemical and molecular genetic study. Hum Reprod 2006 May 12
• Steppan CM, Bailley ST, Brown EJ, Banerjee RR, Wright CM, Patel HR, Ahima RS, Lazar MA: The Hormone Links Obesity to Diabetes. Nature. 409, 307-312, (2001)
• Hartman HB, Hu X, Tyler KX, Dalal CK, Lazar MA: Mechanisms Regulating Adipocyte Expression of Resistin. J Biol Chem. 277, 19754-19761, (2002)
• Steppan CM, Brown EJ, Wright CM, Bhat S, Banerjee RR, Dai CY, Enders GH, Silberg DG, Wen X, Wu GD, Lazar MA: A Family of Tissue-specific Resistin-like Molecules. PNAS. 98, 502-506, (2001)
• Way JM, G rg n CZ, Tong Q, Uysal KT, Brown KK, Harrington WW, Oliver WR Jr., Willson TM, Kliewer SA, Hotamisligil GS: Adipose Tissue Resistin Expression Is Severly Suppressed in Obesity and Stimulated by Peroxisome Proliferator-activated Receptor Agonists. J Biol Chem. 276, 25651-25653, (2001)
• Banerjee RR, Lazar MA: Dimerization of resistin and resistin–like moleules is etermined by a single cystein. J Biol Chem. 276, 25970-25973, (2001)
• Steppan CM, Lazar MA: Resistin and obesity-associated insulin resistance. TRENDS Endocrinol Metab. 13, 18-23, (2002)
• Pravenec M, Kazdov L, Landa V, Z dek V, Mlejnek P, Jansa P, Wang J, Qi N, Kurtz TW: Transgenic and recombinant resistin impair skeletal muscle glucose metabolism in the spontaneously hypertensive rat. J Biol Chem. 278, 45209-45215, (2001)