Technical Data
R1585-09P
Resistin (ADSF, Adipose Tissue-Specific Secretory Factor, FIZZ3, Found in Inflammatory Zone)
Description:
Resistin belongs to a family of tissue-specific cytokines termed FIZZ (found in inflammatory zones) and RELM. The three known members of this family (Resistin, RELM-alpha and RELM-beta) share a highly conserved C-terminal domain, characterized by 10 cysteine residues with a unique spacing motif of C-X11-C-X8-C-X-C-X3-C-X10-C-X-C-X-C-X9-C-C. Resistin is an adipose-derived cytokine (adipokine) whose physiological function and molecular targets are largely unknown. Studies have shown that Resistin suppresses insulin's ability to stimulate glucose uptake. It is postulated that Resistin might be an important link between obesity and Type 2 diabetes. Other studies have indicated that Resistin expression is severely suppressed in obesity and that it may act as a feedback regulator of Adipogenesis. Recombinant human Resistin is a 19.5kD disulfide-linked homodimeric protein composed of two identical 92 amino acid chains linked by a single disulfide bond.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA (Direct and indirect): 1:5000-1:10,000
Western Blot: 1:2000-1:10,000 (ECL); Suggested blocking and dilution buffer is TBST containing 0.05% Tween-20 and 5% skim milk. Suggested incubation time is 1 hour at RT.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a6D730Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant rat resistin.
Purity:
Purified by Protein A/G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4. No preservative added.
Specificity:
Recognizes both natural and recombinant rat resistin. Detects a band of ~10kD by Western Blot. Species Crossreactivity: Weakly crossreacts with human and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.