Technical Data
R1585-09T
Resistin (ADSF, Adipose Tissue-Specific Secretory Factor, FIZZ3, Found in Inflammatory Zone)
Description:
Resistin belongs to a family of tissue-specific cytokines termed FIZZ (found in inflammatory zones) and RELM. The three known members of this family (Resistin, RELM-alpha and RELM-beta) share a highly conserved C-terminal domain, characterized by 10 cysteine residues with a unique spacing motif of C-X11-C-X8-C-X-C-X3-C-X10-C-X-C-X-C-X9-C-C. Resistin is an adipose-derived cytokine (adipokine) whose physiological function and molecular targets are largely unknown. Studies have shown that Resistin suppresses insulin's ability to stimulate glucose uptake. It is postulated that Resistin might be an important link between obesity and Type 2 diabetes. Other studies have indicated that Resistin expression is severely suppressed in obesity and that it may act as a feedback regulator of Adipogenesis. Recombinant human Resistin is a 19.5kD disulfide-linked homodimeric protein composed of two identical 92 amino acid chains linked by a single disulfide bond.

Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteine-rich secreted proteins which is termed the RELM family, and is also described as ADSF (Adipose Tissue-Specific Secretory Factor) and FIZZ3 (Found in Inflammatory Zone). Human resistin contains 108 amino acids as a prepeptide, and its hydrophobic signal peptide is cleaved before its secretion. Resistin circulates in human blood as a dimeric protein consisting of two 92 amino acid polypeptides, which are disulfide-linked via Cys26.

Resistin may be an important link between obesity and insulin resistance. Mouse resistin, specifically produced and secreted by adipocyte, acts on skeletal muscle myocytes, hepatocytes and adipocytes themselves so that it reduces their sensitivity to insulin. Steppan et al. have suggested that resistin suppresses the ability of insulin to stimulate glucose uptake. They have also suggested that resistin is present at elevated levels in blood of obese mice, and is down regulated by fasting and antidiabetic drugs. Way et al., on the other hand, have found that resistin expression is severly suppressed in obesity and is stimulated by several antidiabetic drugs. Other studies have shown that mouse resistin increases during the differentiation of adipocytes, but it also seems to inhibit adipogenesis. In contrast, the human adipogenic differentiation is likely to be associated with a down regulation of resistin gene expression.

Applications:
Suitable for use in ELISA and Western Blot. Other applications have not been tested.

Recommended Dilution:
ELISA (direct and indirect): 1:5000-1:10,000
Western Blot: 1:2000-1:10000 using ECL.
Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% non-fat milk. Suggested incubation time is 1 hour at RT).
Optimal conditions must be determined individually for each application.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG16D731Purified
SizeStorageShippingSourceHost
50ug4C (-20C Glycerol)Blue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant rat resistin:Fc expressed from mammalian cells
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.4. No preservative added.
Specificity:
Recognizes both natural and recombinant rat resistin at ~10kD. Does not cross-react with human and mouse resistin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.