Technical Data
R1585-21C2
Resistin (RSTN, FIZZ3, ADSF, Adipose Tissue-Specific Secretory Factor)
Description:
Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteine-rich secreted proteins which is termed the RELM family, and is also described as ADSF (Adipose Tissue-Specific Secretory Factor) and FIZZ3 (Found in Inflammatory Zone). Human resistin contains 108 amino acids as a prepeptide, and its hydrofobic signal peptide is cleaved before its secretion. Resistin circulates in human blood as a dimeric protein consisting of two 92 amino acid polypeptides, which are disulfide-linked via Cys26. Resistin may be an important link between obesity and insulin resistance. Mouse resistin, specifically produced and secreted by
adipocyte, acts on skeletal muscle myocytes, hepatocytes and adipocytes themselves so that it reduces their sensitivity to insulin. Steppan et al. have suggested that resistin suppresses the ability of insulin to stimulace glucose uptake. They have also suggested that resistin is present at elevated levels in blood of obese mice, and is down regulated by fasting and antidiabetic drugs. Way et al., on the other hand, have found that resistin expression is severly suppressed in obesity and is stimulated by several antidiabetic drugs. Other studies have shown that mouse resistin increases during the differentiation of adipocytes, but it also seems to inhibit adipogenesis. In contrast, the human adipogenic differentiation is likely to be associated with a down regulation of resistin gene expression. Recent studies have shown that human resistin is expressed also in macrophages and may be a novel link between inflammation and insulin resistance.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stabillity:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabigGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Recombinant protein corresponding to human Resistin expressed in E. coli
Purity:
Purified by Immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.2. No preservative added. Reconstitute with 100ul sterile ddH2O.
Specificity:
Recognizes human Resistin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Pravenec M, Kazdova L, Cahova M, Landa V, Zidek V, Mlejnek P, Simakova M, Wang J, Qi N, Kurtz TW . Fat-specific transgenic expression of resistin in the spontaneously hypertensive rat impairs fatty acid re-esterification. Int J Obes (Lond) . Jul;30(7):1157-9 (2006). 2. Senolt L, Housa D, Vernerova Z, Jirasek T, Svobodova R, Veigl D, Anderlova K, Muller-Ladner U, Pavelka K, Haluzik M . Resistin in rheumatoid arthritis synovial tissue, synovial fluid and serum. Ann Rheum Dis. Apr;66(4):458-63 (2007). 3. Senolt L, Housa D, Vernerova Z, Jirasek T, Svobodova R, Veigl D, Anderlova K, Muller-Ladner U, Pavelka K, Haluzik M . Resistin is abundantly present in rheumatoid arthritis synovial tissue, synovial fluid, and elevated serum resistin reflects disease activity. Ann Rheum Dis. Nov 22 (2006). 4. Reverchon M, Cornuau M, Rame C, Guerif F, Royere D, Dupont J. Resistin decreases insulin-like growth factor I-induced steroid production and insulin-like growth factor I receptor signaling in human granulosa cells. Fertil Steril. 2013 Jul;100 (1):247-55.e1-3