Technical Data
R1585-21C4
Resistin (FIZZ-3, RSTN, FIZZ3, ADSF, Adipose Tissue-Specific Secretory Factor)
Description:
Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteine-rich secreted proteins which is termed the RELM family, and is also described as ADSF (Adipose Tissue-Specific Secretory Factor) and FIZZ3 (Found in Inflammatory Zone). Human resistin contains 108 amino acids as a prepeptide, and its hydrofobic signal peptide is cleaved before its secretion. Resistin circulates in human blood as a dimeric protein consisting of two 92 amino acid polypeptides, which are disulfide-linked via Cys26. Resistin may be an important link between obesity and insulin resistance. Mouse resistin, specifically produced and secreted by
adipocyte, acts on skeletal muscle myocytes, hepatocytes and adipocytes themselves so that it reduces their sensitivity to insulin. Steppan et al. have suggested that resistin suppresses the ability of insulin to stimulace glucose uptake. They have also suggested that resistin is present at elevated levels in blood of obese mice, and is down regulated by fasting and antidiabetic drugs. Way et al., on the other hand, have found that resistin expression is severly suppressed in obesity and is stimulated by several antidiabetic drugs. Other studies have shown that mouse resistin increases during the differentiation of adipocytes, but it also seems to inhibit
adipogenesis. In contrast, the human adipogenic differentiation is likely to be associated with a down regulation of resistin gene expression. Recent studies have shown that human resistin is expressed also in macrophages and may be a novel link between inflammation and insulin resistance.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stabillty:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceRabbitRat
Concentration:
~1mg/ml
Immunogen:
Recombinant rat Resistin expressed in E. coli
Purity:
Purified by Immunoaffinity chromatography.
Form
Supplied as a lyophilized powder in PBS, pH 7.2. No preservative added. Reconstitute with 100ul sterile dH2O. Let the lyophilized pellet dissolve completely.
Specificity:
Recognizes rat Resistin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kim KH. et al. A Cysteine-rich Adipose Tissue-specific Secretory Factors Inhibits Adipocte
Differentiation. The Journal of Biological Chemistry. 276 (14), 11252-11256, (2001) .
2. Steppan CM, Brown EJ, Wright CM, Bhat S, Banerjee RR, Dai CY, Enders GH, Silberg DG, Wen X,
Wu GD, Lazar MA: . A Family of Tissue-specific Resistin-like Molecules. PNAS. 98, 502-506,
(2001) .
3. Steppan C.M. at al. A Family of Tissue-specific Resistin-like Molecules. Proc. Natl. Acad. Sci.
USA. 98 (2), 502-506, (2001) .
4. Way J.M. et al. Adipose Tissue Resistin Expression Is Severly Suppressed in Obesity and
Stimulated by Peroxisome Proliferator-activated Receptor g Agonists. The Journal of Biological
Chemistry. 276 (28), 25651-25653, (2001) .
5. Way JM, Görgün CZ, Tong Q, Uysal KT, Brown KK, Harrington WW, Oliver WR Jr., Willson TM, Kliewe
r
SA, Hotamisligil GS: . Adipose Tissue Resistin Expression Is Severly Suppressed in Obesity and
Stimulated by Peroxisome Proliferator-activated Receptor ? Agonists. J Biol Chem. 276, 25651-
25653, (2001) .
6. Banerjee RR, Lazar MA: . Dimerization of resistin and resistin-like moleules is etermined by a
single cystein. J Biol Chem. 276, 25970-25973, (2001) .
7. Banerjee R.R., Lazar M.A. Dimerization of resistin and resistin-like moleules is etermined by a
single cystein. The Journal of Biological Chemistry. 276, 25970-25973, (2001) .
8. Maebuchi M, Machidori M, Urade R, Ogawa T, Moriyama T: . Low resistin levels in adipose
tissues and serum high-fat fed mice and genetically obese mice: development of an ELISA
system for quantification of resistin. Archiv Biochem Biophys. 416, 164-170 (2003) .
9. Hartman HB, Hu X, Tyler KX, Dalal CK, Lazar MA: . Mechanisms Regulating Adipocyte
Expression of Resistin. J Biol Chem. 277, 19754-19761, (2002) .
Page 2 of 3 (VERSION: 2009-10-22)
10. Hartman H.B., Lazar M.A. at al. Mechanisms Regulating Adipocyte Expression of Resistin. The
Journal of Biological Chemistry. 277 (22), 19754-19761, (2002) .
11. Steppan CM, Lazar MA: . Resistin and obesity-associated insulin resistance. TRENDS
Endocrinol Metab. 13, 18-23, (2002) .
12. Steppan C.M., Lazar M.A. Resistin and obesity-associated insulin resistance. TRENDS in
Endocrinology and Metabolism. 13 (1), 18-23, (2002) .
13. Juan CC. at al. Suppressed Gene Expression of Adipocyte Resistin in an Insulin-resistant Rat
Model Probably by Elevated Free Fatty Acids. Biochemical and Biophysical Research
Communications. 289, 1328-1333, (2001) .
14. Steppan CM, Bailley ST, Brown EJ, Banerjee RR, Wright CM, Patel HR, Ahima RS, Lazar MA: . The
Hormone Links Obesity to Diabetes. Nature. 409, 307-312, (2001) .
15. Steppan C.M. et al. The Hormone Links Obesity to Diabetes. Nature. 409, 307-312, (2001) .
16. Pravenec M, Kazdová L, Landa V, Zídek V, Mlejnek P, Jansa P, Wang J, Qi N, Kurtz TW: .
Transgenic and recombinant resistin impair skeletal muscle glucose metabolism in the
spontaneously hypertensive rat. J Biol Chem. 278, 45209-45215, (2001) .
17. Fasshauer M. at al. Tumor Necrosis Factor a Is a Negative Regulator of Resistin Gene
Expression and Secretion in 3T3-L1 Adipocytes. Biochemical and Biophysical Research
Communications. 208, 1027-1031, (2001) .