Technical Data
R1585-21D
Resistin (ADSF, Adipose Tissue-Specific Secretory Factor, FIZZ3, Found in Inflammatory Zone)
Description:
A family of resistin-like molecules (RELMs) has been identi ed in rodents and humans. Resistin is a hormone produced by fat cells. RELMalpha is a secreted protein that has a restricted tissue distribution with highest levels in adipose tissue. Another family member, RELMbeta, is a secreted protein expressed only in the gastrointestinal tract, particularly the colon, in both mouse and human. RELMbeta gene expression is highest in proliferative epithelial cells and is markedly increased in tumors, suggesting a role in intestinal proliferation. Resistin and the RELMs share a cysteine composition and other signature features. Thus, the RELMs together with resistin comprise a class of tissue-speci c signaling molecules.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA (Sandwich): 0.5ug/ml will detect at least 0.1ng/well of recombinant human Resistin when used with R1585-21A1 as the detection antibody at a concentration of 0.5ug/ml.
Western Blot: 0.2-0.4ug/ml. When used in conjuction with compatible secondary reagents, the detection limit for rhResistin is 0.2-0.4ng/lane under non-reducing conditions.
Immunohistochemistry (FFPE): 1ug/ml using human normal placenta with a two hour incubation at RT. Heat induced antigen retrieval with sodium citrate buffer, pH 6.0 is recommended.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b,k7F8Affinity Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Recombinant protein corresponding to human Resistin expressed in E. coli.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a lyophilized powder from PBS .Reconstitute with 500ul sterile ddH2O.
Specificity:
Recognizes human Resistin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.