Resistin-like Molecule, beta, Recombinant, Human (RELMb)
|Growth Factors, Cytokines||Storage: -20°CShipping: Blue Ice|
RELM-beta (Resistin-Like Molecule-beta) is a member of a recently identified family of secreted proteins containing a conserved cystein-rich C-terminus. The RELM family consists of resistin (also called FIZZ3), RELM-alfa (FIZZ1), RELM-beta (FIZZ2) and RELM-gamma. Only resisistin and RELM-beta were found in humans whereas all four RELM family members were identified in rodents. RELM-beta appears to be produced as a homodimer exclusively by intestinal goblet cells and can be found in high quantities in stool. Remarkably, stool of germ-free mice displaying sterile intestinal tract does not contain RELM-beta until bacterial colonization takes place after pathogen-free mice entered natural environment. Some, but not all, colon carcinoma cell lines secrete RELM-beta into the cell culture supernatant. The physiological function of RELM-beta is not known. High doses of recombinant RELM-beta showed hyperglycemic effects including lowered glucose disposal and increased hepatic glucose production in mice.
Recombinant Human RELM-beta is a His-Tagged Fusion Protein, 11kD protein containing 90 amino acid residues of the human RELM-beta and 12 additional amino acid residues-HisTag (underlined).
Amino Acid Sequence:
MG STQCSLDS VMDKKIKDVL NSLEYSPSPI SKKLSCASVK SQGRPSSCPA GMAVTGCACG YGCGSWDVQLETTCHCQCSV VDWTTARCCH LTKLRSHHHH HH.
The amino acid sequence of the recombinant human RELM-beta is 100% homologous to the amino acid sequence of the human RELM-beta without signal sequence.
Western blotting, ELISA
Add 0.2 ml of 0.1M Acetate buffer pH4 and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 g/ml. In higher concentrations the solubility of this antigen is limited.
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. The lyophilized protein remains stable until the expiry date when stored at -20°C.
Source: E. coli
Purity: 95% (SDS-PAGE); Three-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding.
Form: Supplied as a lyophilized powder in 0.05M acetate buffer, pH 4.0.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Human RELMbeta is a mitogenic factor in lung cells and induced in hypoxia.
FEBS Lett 2006 Feb 6;580(3):900-3
2. RELMbeta/FIZZ2 is a goblet cell-specific immune-effector molecule in the gastrointestinal tract.
Proc Natl Acad Sci U S A 2004 Sep 14;101(37):13596-600
3. Bacterial colonization leads to the colonic secretion of RELMbeta/FIZZ2, a novel goblet cell-specific protein.
Gastroenterology 2003 Nov;125(5):1388-97