Technical Data
Respiratory Syncytial Virus (RSV)
Respiratory syncytial virus (RSV) is the most significant respiratory pathogen of early development and causes about half of all cases of bronchiolitis and a quarter of all cases of pneumonia during the first few months of life. There are multiple types and subtypes of RSV. VP-R151 is a mixture of four antibodies to optimize sensitivity and detection of the various RSV forms.

Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunofluorescence: 1:10
Immunohistochemistry: 1:200-1:400 for 1 hr at RT Requires antigen retrieval.
Optimal dilutions to be determined by the researcher.

Positive Control:
Indirect immunofluorescence: Acetone-fixed HeLa cells infected with RSV-A2. Staining pattern RSV-infected epithelial cell membranes and cytoplasm.
Immunohistochemistry: Formalin-fixed, paraffin-embedded RSV infected lung.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG1, IgG2a5H5N, 2G122, 5A6, IC3Supernatant
1ml-20CBlue IceMouse
Not Determined
Respiratory syncytial virus strain A2.
Supplied as lyophilized powder with 15mM sodium azide. Reconstitute with 1ml sterile ddH2O.
Recognizes Respiratory Syncytial Virus. Does not crossreact with tissue culture isolates of influenza virus types A and B, parainfluenza virus types 1, 2, 3 and 4b, adenovirus, herpes simplex virus types 1 and 2, varicella-zoster virus, cytomegalovirus, mumps virus, measles virus, ECHOvirus 19, coxsackie B4 virus, poliovirus types 1, 2 and 3 or negative tissue culture cells used in routine virus isolation.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Wright C, Oliver K C, Fenwick F, et al.. A monoclonal antibody pool for routine immunohistochemical detection of human respiratory syncytial virus anti- gens in formalin-fixed, paraffin-embedded tissue. Journal of Pathology. 182:238-244 (1997). 2.Routledge E C, McQuillan J, Samson A C R, et al.. The development of monoclonal antibodies to respiratory syncytial virus and their use in diagnosis by indirect immunofluorescence. Journal of Medical Virology. 15:305-320 (1985).