Technical Data
R1600-13C
Respiratory Syncytial Virus (RSV)
Description:
Respiratory syncytial virus (RSV) is the most significant respiratory pathogen of early development and causes about half of all cases of bronchiolitis and a quarter of all cases of pneumonia during the first few months of life. There are multiple types and subtypes of RSV. VP-R151 is a mixture of four antibodies to optimize sensitivity and detection of the various RSV forms.

Applications:
Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunofluorescence: 1:10
Immunohistochemistry: 1:200-1:400 for 1 hr at RT Requires antigen retrieval.
Optimal dilutions to be determined by the researcher.

Positive Control:
Indirect immunofluorescence: Acetone-fixed HeLa cells infected with RSV-A2. Staining pattern RSV-infected epithelial cell membranes and cytoplasm.
Immunohistochemistry: Formalin-fixed, paraffin-embedded RSV infected lung.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1+IgG2a5H5N, 2G122, 5A6, IC3Supernatant
SizeStorageShippingSourceHost
1ml-20CBlue IceMouse
Concentration:
Not Determined
Immunogen:
Respiratory syncytial virus strain A2.
Purity:
Supernatant
Form
Supplied as lyophilized powder with 15mM sodium azide. Reconstitute with 1ml sterile ddH2O.
Specificity:
Recognizes Respiratory Syncytial Virus. Does not crossreact with tissue culture isolates of influenza virus types A and B, parainfluenza virus types 1, 2, 3 and 4b, adenovirus, herpes simplex virus types 1 and 2, varicella-zoster virus, cytomegalovirus, mumps virus, measles virus, ECHOvirus 19, coxsackie B4 virus, poliovirus types 1, 2 and 3 or negative tissue culture cells used in routine virus isolation.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Wright C, Oliver K C, Fenwick F, et al.. A monoclonal antibody pool for routine immunohistochemical detection of human respiratory syncytial virus anti- gens in formalin-fixed, paraffin-embedded tissue. Journal of Pathology. 182:238-244 (1997). 2.Routledge E C, McQuillan J, Samson A C R, et al.. The development of monoclonal antibodies to respiratory syncytial virus and their use in diagnosis by indirect immunofluorescence. Journal of Medical Virology. 15:305-320 (1985).