Technical Data
R1992-05
Reverse Transcriptase, HIV-1 (Human Immunodeficiency Virus-1 RT)
Description:
Applications:
Suitable for use in ELISA, Western Blot, Conjugation, Immunohistochemistry, Immunocytochemistry and Immunoadsorbance. Other applications have not been tested.

Recommended Dilution:
Conjugation: Enzymes, radiolabels, fluorchromes and other markers.
Optimal dilutions to be determined by the researcher.

Source:
Produced by murine hybridoma cells cultured in protein free medium in hollow fiber systems.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
TypeIsotypeCloneGrade
MabIgG12W1Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceMouse
Concentration:
~0.2mg/ml (after reconstitution)
Immunogen:
Synthetic peptide corresponding to aa387-412 of Reverse Transcriptase, HIV-1 (Human Immunodeficiency Virus-1 RT).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a lyophilized powder from 10mM Tris HCl, pH 7.4, 15% sucrose. No preservative added. Reconstitute with 500ul sterile ddH2O.
Specificity:
Recognizes aa387-412 of Reverse Transcriptase, HIV-1 (Human Immunodeficiency Virus-1 RT). Does not crossreact to HIV-2. The immunoactivity and specificity are tested in ELISA using viral lysate and recombinant proteins. The specificity is confirmed by Western Blot (pattern p51 and p65).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.