Technical Data
RhoA,B,C (GDP/GTP Binding Protein)
Suitable for use in Immunoblot, Immunocytochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Immunoblot: 0.5-3ug/ml detects Rho in RIPA lysates from Jurkat and HL-60 cells. HL-60 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with 3ug/ml R1998-01. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunocytochemistry: Reported to detect Rho in cells via immunofluorescence.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG14F5Affinity Purified
200ug4C (-20C Glycerol)Blue IceHumanMouse
Recombinant human RhoA containing amino acids 1-155.
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, 0.09% sodium azide, before the addition of glycerol to 30%.
Recognizes human Rho (-A, -B, -C), Mr 22kD. Species Crossreactivity: rat and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Arthur, W.T., et al., J. Biol. Chem. 277: 42964-42972, 2002. 2. DerMardirossian, C. and Bokoch, G.M., Drug News Perspect., 14: 389-395, 2001. 3. Symons, M. and Rusk, N., Curr. Biol., 13: 409-418, 2003.
4. Raftopoulou, M. and Hall, A., Dev. Biol., 265: 23-32, 2004.