Suitable for use in Immunoblot, Immunocytochemistry and Immunofluorescence. Other applications not tested.
Western Blot: 1:1000-1:4000 detects Rho (-A, -B, -C) in RIPA lysates from human HL-60 and rat PC-12 cells. HL-60 cell lysate was resolved by electrophoresis, transferred to nitrocellulose
and probed with anti-Rho (-A, -B, -C), (1:2000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
| Not determined|
|KLH-conjugated, synthetic peptide (SYPDTDVILMCFSIDSPDSLENKK) corresponding to amino acids 73-94 of human Rho (-A, -B, -C), with two terminal lysines added to increase peptide solubility.|
|Purified by Protein A affinity chromatography.|
|Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.|
|Recognizes human Rho (-A, -B, -C), Mr 22kD. Recognizes recombinant RhoA protein, but not recombinant rac1 or cdc42 proteins. Species Crossreactivity: Rat. Broadspecies cross-reactivity expected based on sequence homology.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
Denhardt, D.T., Biochem. J. 318: 729-747, 1996. Jiang, W.G., et al., Clin. Cancer Res. 9: 6432-6440, 2003. Vincent, S. and J. Settleman, Mol. Cell. Biol. 17: 2247-2256, 1997. 1. Arthur, W.T., et al., J. Biol. Chem. 277: 42964-42972, 2002. 2. DerMardirossian, C. and Bokoch, G.M., Drug News Perspect., 14: 389-395, 2001. 3. Symons, M. and Rusk, N., Curr. Biol., 13: 409-418, 2003. 4. Raftopoulou, M. and Hall, A., Dev. Biol., 265: 23-32, 2004.|