Technical Data
ARHGEF11 (Rho Guanine Nucleotide Exchange Factor 11, PDZ-RhoGEF, KIAA0380)
ARHGEF11 is a 1527aa protein with tandem dbl homology and pleckstrin homology domains, PDZ domain, two proline rich sequences and a regulatory G protein-signaling (RGS) sequence. It acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase, but not for RAC1 or CDC42 and acts as GTPase-activating protein (GAP) for GNA12 and GNA13. It forms a complex with G proteins and stimulates neurite through retraction Rho-dependent signals. ARHGEF11 increases glutamate transport 4-fold. It interacts with EAAT4 and modulates its uptake activity and perisynaptic distribution at glutamatergic synapses. It is known to induce the reorganization of the actin cytoskeleton and its overexpression induces the formation of membrane ruffling and filopodia. It is ubiquitously expressed, in brain, lung, liver, kidney and skeletal muscle.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1-0.5ug/ml
Immunohistochemistry (paraffin): 10ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Rat brain lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
Synthetic peptide corresponding to aa1-50 of human ARHGEF11.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Recognizes human ARHGEF11. Species Crossreactivity: chimpanzee, bovine, monkey. Species sequence homology: mouse, rat, canine, chicken, equine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Jackson, M. et al. Nature. 410:89-93 (2001). 2. Banerjee, J. et al. Mol. Biol. Cell. 15:1760-1775 (2004).