Technical Data
R1998-35
Rho, NT
Description:
Rho belongs to the Ras super family of low molecular weight GTPases. Fifteen Rho proteins have already been characterized and divided into three sub-families; the first includes Rho (A, B, C), the second, Rnd 1-3 and the third which includes Rac 1-3, RhoG, Cdc42Hs, Rho/TTF. TC10 and Chp. Rho acts as a molecular switch which turns on of off various intracellular signaling pathways such as ACK, PAKs, MEKKs ROCK (3). Rho is active when bound to GTP and inactive when bound to GDP. It is also known to participate in many physiological activities including cell migration, adhesion, cytokinesis, proliferation, differentiation and apoptosis and to a greater extend cell transformation.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Not recommended for use in Flow Cytometry or Immunoprecipitation.Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000-1;10,000 detects a band at ~22kD
Immunohistochemistry (Paraffin): 1:50-1:100
Immunocytochemistry (IF): 1:100-1:250
Optimal dilutions to be determined by the researcher.

Positive Controls:
PC12 cell lysate and hepatocellular carcinoma

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG9E528Purified
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
A synthetic peptide corresponding to residues in the N-terminal of human Rho. Cellular Localization: Cell membrane, Cytoplasm
Purity:
Purified
Form
Supplied as a liquid in PBS,, pH 7.4, 0.05% BSA, 0.01% sodium azide, 50% glucerol
Specificity:
Recognizes human Rho, NT. Species Crossreactivity: mouse and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Murphy et al. Nature. 384:427-432 2. Yeramian P, Chardin P, Madaule P, Tavitian A. Nucleic Acids Res. 1987 Feb 25;15(4):1869. 3. Maekawa et al. Science 285: 895-898, 1999. 4. Boguski, M.S., and F. McCormick. 1993. Nature. 366:643- 654 5. Khosravi-Far, R., P.A. Solski, G.J. Clark, M.S. Kinch, and C.J. Der. 1995. Mol. Cell. Biol. 15:6443-6453.