Technical Data
R2011-13
Ribonuclease A (RNase A, Pancreatic Ribonuclease, Rib 1, Ribonuclease A Family 1, Ribonuclease Pancreatic, Ribonuclease RNase A Family 1, Ribonuclease RNase A Family 1 Pancreatic, RNAseA, RNase 1, RNASE1, RNS1)
Description:
Ribonuclease A is used to isolate DNA for RNA-free DNA. Ribonuclease A hydrolyzes RNA at C and U residues, cleaving between the 3-Phosphate group of a pyrimidine nucleotide and the 5-hydroxyl group of the adjacent nucleotide, i.e., those cleaving cyclic 2',3'-pyrimidine nucleotide residues either singly or at the termination of purine nucleotide chains (Volkin and Cohn 1953). The composition and structure of ribonuclease A has been extensively investigated. The tertiary structure was reported by Kartha, et al. (1967) and Lin, (1970). Wang, et al. (1975) indicate that flexibility in a three-dimensional structure is essential for optimal catalytic activity. Ribonuclease is inhibited by heavy metal ions and is competitively inhibited by DNA. The effect of denatured DNA is much greater than that of the native nucleic acid (Sekine et al. 1969).

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Conjugation. Other applications not tested.

Recommended Dilution:
ELISA: 1:40,000-1:80,000 use 1ug of R2011-13 in a standard sandwich ELISA using I1904-46Q and ABTS as a substrate for 30 min at RT.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
10mg4C (-20C Glycerol)Blue IceBovineRabbit
Concentration:
~10mg/ml (UV280)
Immunogen:
Ribonuclease A (Bovine pancreas)
Purity:
Puri ed by ion exchange chromatography.
Form
Supplied as a lyophilized powder in PBS, pH 7.2, 0.01% sodium azide. Reconstitute with sterile ddH2O.
Specificity:
Recognizes bovine Ribonuclease A. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti rabbit serum as well as purified and partially purified ribonuclease A (bovine pancreas).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.