Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry. Other applications not tested.
Western Blot: Alkaline phosphatase/ECL detection. Detects a band at ~83kD
Immunohistochemistry: Paraffin sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:
For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|100ug||4°C (-20°C Glycerol)||Blue Ice||Human||Mouse|
|FPLC purified recombinant human RNase L produced in insect cells|
|Purified by affinity chromatography.|
|Supplied as a Liquid in PBS, containing 10% glycerol and 0.02% sodium azide.|
|Recognizes human RNase L. Does not cross-react with mouse RNase L.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Dong B and Silverman RH. 1995. J. Biol. Chem. 270:4133. 2. Wang L, et al. 1995. Clin. Cancer Res. 1:1421. 3. Dong B and Silverman RH. 1999. Nucleic Acids Res. 27:439. 4. Carpten J, et al. 2002. Nat. Genet. 30:181. 5. Silverman RH. 2003. Biochemistry 42:1805. 6. Xiang Y, et al. 2003. Cancer Res. 63:6795|