Technical Data
R2027-90
Ribonuclease P Protein Subunit p20 (RNaseP Protein p20, RPP20, 0610037N12Rik, hPOP7, Processing of Precursor 7 Ribonuclease P/MRP Subunit (S. cerevisiae), POP7, Ribonucleases P/MRP Protein Subunit POP7 Homolog, RPP2)
Description:
The nuclei of eukaryotic cells contain several classes of small RNA-protein complexes. SnRNPs (small nuclear ribonucleoproteins) are involved in splicing of pre-mRNAs (the excision of introns) in the nucleoplasm. RPP20 is part of such a complex. SnoRNPs (small nucleolar ribonucleoproteins) are involved in the maturation of pre-ribosomal RNA in the nucleoli. Pre-rRNA processing and modification require both the snoRNA and the protein constituents (such as Rpp20) of these complexes. Generally, snoRNPs contain a number of common proteins, which are shared with other snoRNPs of the same family, next to several particle- specific proteins. On average, snoRNPs contain about 6-10 protein subunits.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: Under less stringent washing conditions, a weaker band of ~60kD is also detected.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a5BH5Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
Not determined.
Immunogen:
Recombinant GST-hRPP20 (Accession #AAC24113, expression vector pGEX-2T), expressed in E.coli.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a lyophilized powder from 10mM ammonium bicarbonate buffer, 2mg BSA. Reconstitute with sterile dH2O.
Specificity:
Recognizes human Ribonuclease P Protein Subunit p20.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Welting et al. Autoantigenicity of nucleolar complexes. Autoimmun Rev. 2003 Oct;2(6):313-21. 2. Van Eenennaam et al. Architecture and function of the human endonucleases RNase P and RNase MRP. IUBMB Life. 2000 Apr;49(4):265-72. 3. Welting et al. Mutual interactions between subunits of the human RNase MRP ribonucleoprotein complex. Nucleic Acids Res. 2004 Apr 19;32(7):2138-46. Print 2004.