Technical Data
R2031-38J
RPS6KB1 (Ser424) (Ribosomal Protein S6 Kinase beta-1, S6K-beta-1, S6K1, 70kD Ribosomal Protein S6 Kinase 1, P70S6K1, p70-S6K 1, p70 Ribosomal S6 Kinase alpha, p70 S6 Kinase alpha, p70 S6K-alpha, p70 S6KA, p70-S6K, Ribosomal Protein S6 Kinase I, Serine/thr
Description:
RPS6KB1 is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding the gene for RPS6KB1 and overexpression of this kinase are seen in some breast cancer cell lines.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to amino acid residues surrounding S424 of human RPS6KB1 (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human RPS6KB1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Adem, C., et al., Genes Chromosomes Cancer 41(1):1-11 (2004). 2.Suzuki, Y., et al., Genome Res. 14(9):1711-1718 (2004). 3.Gomez-Cambronero, J., et al., Leuk. Res. 28(7):755-762 (2004). 4.Raught, B., et al., EMBO J. 23(8):1761-1769 (2004). 5.Miyakawa, M., et al., Endocr. J. 50(1):77-83 (2003).