Technical Data
R3000-01A
Rotavirus, ICP, Late Structural Virion Antigens
Description:
Rotaviruses, members of the family Reoviridae, are a major cause of diarrhea in young mammals. Human rotaviruses, the major etiological agents of severe infantile diarrhea worldwide, display very diverse and complex serotypic specificities. Rotaviruses are 70nm, non enveloped viruses comprised of a triple layered protein capsid; Outer capsid proteins are VP4 and VP7, Inner capsid -VP6 and Core -VP2. The immunity acquired from exposure to rotavirus appears to be type specific following initial infection. Therefore, multiple serotypes of rotavirus mean multiple opportunities for infection. The combination of animal reservoirs for the virus and rotavirus gene reassortment provides the potential for dramatic genetic shifts (similar to influenza virus) which could give rise to altered host ranges and viral virulence.

Applications:
Suitable for use in Indirect Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Indirect Immunofluorescence: ~1:250
Western Blot: Unboiled samples in 1mM DTT and 2% SDS only.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
1ml-20CBlue IceGoat
Concentration:
Not Determined
Immunogen:
Bovine (Nebraska Calf Diarrhea Virus)
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Recognizes Rotavirus (ICPs and late structural virion antigens). Species Crossreactivity: >90% reactive with human rotaviruses. Non-reactive with HEp-2 cells and WI-38 cells by indirect immunofluorescence.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.