Technical Data
R3000-01F
Rotavirus, ICP, Late Structural Virion Antigens (FITC)
Description:
Applications:
Suitable for use in Immunofluorescence. Other applications not tested.

Recommended Dilutions:
Immunofluorescence: 1:10-1:50. Direct FA staining of target antigens in a permissive tissue culture system. Acetone fixation of the antigen source is recommended prior to staining.
Optimal dilutions to be determined by the researcher.

Titer:
~1:250 by Indirect Immunofluorescence

Storage and Stability:
Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
1ml-20CBlue IceGoat
Concentration:
~4-5mg/ml
Immunogen:
Bovine (Nebraska Calf Diarrhea Virus)
Purity:
Purified IgG fraction to a purity of > 95%.
Form
Supplied as a liquid in 0.01M PBS, pH 7.2, 10mg/ml BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Specificity:
Recognizes ICPs and late structural (virion) antigens. Reacts >90% with human rotaviruses. Uninfected Cell Reactivity: Negative vs. HEp-2 cells and WI-38 cells by indirect immunofluorescence
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.