Technical Data
R3000-01F
Rotavirus, ICP, Late Structural Virion Antigens (FITC)
Description:
Applications:
Suitable for use in Immunofluorescence. Other applications not tested.

Recommended Dilutions:
Immunofluorescence: 1:10-1:50. Direct FA staining of target antigens in a permissive tissue culture system. Acetone fixation of the antigen source is recommended prior to staining.
Optimal dilutions to be determined by the researcher.

Titer:
~1:250 by Indirect Immunofluorescence

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
1ml-20CBlue IceGoat
Concentration:
~4-5mg/ml
Immunogen:
Bovine (Nebraska Calf Diarrhea Virus)
Purity:
Purified IgG fraction to a purity of > 95%.
Form
Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.1% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Specificity:
Recognizes ICPs and late structural (virion) antigens. Reacts >90% with human rotaviruses. Uninfected Cell Reactivity: Negative vs. HEp-2 cells and WI-38 cells by indirect immunofluorescence
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.