Technical Data
R3000-01G
Rotavirus, ICP, Late Structural Virion Antigens (HRP)
Description:
Rotaviruses, members of the family Reoviridae, are a major cause of diarrhea in young mammals. Human rotaviruses, the major etiological agents of severe infantile diarrhea worldwide, display very diverse and complex serotypic specificities. Rotaviruses are 70nm, non enveloped viruses comprised of a triple layered protein capsid; Outer capsid proteins are VP4 and VP7, Inner capsid -VP6 and Core -VP2. The immunity acquired from exposure to rotavirus appears to be type specific following initial infection. Therefore, multiple serotypes of rotavirus mean multiple opportunities for infection. The combination of animal reservoirs for the virus and rotavirus gene reassortment provides the potential for dramatic genetic shifts (similar to influenza virus) which could give rise to altered host ranges and viral virulence.

Applications:
Suitable for use in ELISA, Immunofluorescence, Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunocytochemistry: 1:20-1:200
Western Blot: 1:20-1:200
ELISA: 1:200-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
1ml-20CBlue IceGoat
Concentration:
~1-2mg/ml
Immunogen:
Bovine (Nebraska Calf Diarrhea Virus).
Purity:
Purified IgG fraction to a purity of > 95%.
Form
Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.002% thimerosal. Labeled with horseradish peroxidase (HRP).
Specificity:
Recognizes ICPs and late structural (virion) antigens. Species Crossreactivity: reported > 90% with human rotaviruses. Uninfected Cell Reactivity: Negative vs. HEp-2 cells and WI-38 cells by indirect immunofluorescence
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.