		Technical Data
R3000-08E	Rotavirus, Capsid Protein

Description:
Rotaviruses, members of the family Reoviridae, are a major cause of diarrhea in young mammals. Rotavirus infections also result in economic losses in agriculture due to diarrhea in calf, pig, sheep, and poultry rearing. Diarrhea (or scours) due to the rotavirus Nebraska Calf Diarrhea Virus can affect calves up to 30 days of age or older. Diarrhea begins 2 to 3 days after exposure. Diagnosis is by history, lesions (ulcers on the tongue, lips, and mouth) and laboratory tests. Mortality rates may be as high as 50 percent, depending on the secondary bacteria present.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 1:20-1:200
Western Blot: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2a	5E422	Affinity Purified

Size		Storage	Shipping	Source	Host
1ml		-20°C	Blue Ice		Mouse

Concentration:

~0.1mg/ml

Immunogen:

Purified bovine rotavirus

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins have been added.

Specificity:

Recognizes the p41 capsid protein. Species Crossreactivity: human, monkey and porcine. Does not react with Influenza A, Influenza B, RSV, Para 1,2 and 3, Adenovirus, M. pneumoniae, H. pylori and mammalian cells.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.