Technical Data
R3000-13
Rotavirus (FITC)
Description:
Rotavirus is a virus that infects the bowels and is the most common cause of gastroenteritis in infants. 7 major groups have currently been identified, 3 of which infect humans (groups A, B and C). Its genome consists of 11 segments of double stranded RNA held in the inner core of the three layered, icosahedral virion particle. The genome codes for 6 virus proteins (VP1,2,3,4,6,7) and 6 non-structural proteins (NSP1-6). Outer capsid proteins are VP4 and VP7, inner capsid -VP6 and core -VP2. The immunity acquired from exposure to rotavirus appears to be type specific following initial infection; therefore, multiple serotypes of rotavirus mean multiple opportunities for infection.

Applications:
Suitable for use in Immunofluorescence. Other applications not tested.

Recommended Dilutions:
Direct Immunofluorescence: 1:20-1:100
Indirect Immunofluorescence:1:500; Acetone fixation of the antigen is recommended.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
1ml-20CBlue IceGoat
Concentration:
~5mg/ml
Immunogen:
Rotavirus (whole virus)
Purity:
Purified by chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.1% sodium azide. Labeled with FITC.
Specificity:
Recognizes Rotavirus (whole virus): 100%
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.