Technical Data
R3000-28
Rotavirus Group Specific Antigen
Description:
Rotaviruses, members of the family Reoviridae, are a major cause of diarrhea in young mammals. Rotavirus infections also result in economic losses in agriculture due to diarrhea in calf, pig, sheep, and poultry rearing. Diarrhea (or scours) due to the rotavirus Nebraska Calf Diarrhea Virus can affect calves up to 30 days of age or older. Diarrhea begins 2 to 3 days after exposure. Diagnosis is by history, lesions (ulcers on the tongue, lips, and mouth) and laboratory tests. Mortality rates may be as high as 50 percent, depending on the secondary bacteria present.

Human rotaviruses, the major etiological agents of severe infantile diarrhea worldwide, display very diverse and complex serotypic specificities. Rotaviruses are 70 nm, non enveloped viruses comprised of a triple layered protein capsid; Outer capsid proteins are VP4 and VP7, Inner capsid -VP6 and Core -VP2. The immunity acquired from exposure to rotavirus appears to be type specific following initial infection. Therefore, multiple serotypes of rotavirus mean multiple opportunities for infection. The combination of animal reservoirs for the virus and rotavirus gene reassortment provides the potential for dramatic genetic shifts (similar to influenza virus) which could give rise to altered host ranges and viral virulence.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 3 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a9C90Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouse
Concentration:
~2mg/ml
Immunogen:
Full length native protein (purified bovine rotavirus, cell culture adapted strain MR).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2.
Specificity:
Rotavirus P42 inner-capsid antigen specific. Crossreacts with monkey rotavirus (SA-11), porcine rotavirus (PP) and human rotaviruses (field strains).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.