Technical Data
R9399-08A
Rsk1, active, Recombinant, Rat (Ribosomal S6 Kinase 1, RSK-1, MAP Kinase-activated Protein Kinase 1a, MAPK-activated Protein Kinase 1a, MAPKAP kinase 1a, Mapkapk1a, MAPKAPK-1a, 90kD Rbosomal Protein S6 Kinase 1, p90RSK1, p90-RSK 1, p90S6K, Ribosomal Prote
10ug
Molecular Biology Storage: -70CShipping: Dry Ice
Recombinant full-length rat RSK1 (residues 1end), containing additional N-terminal 6His and HA (YPYDVPDYA) tags. Expressed in baculovirus in Sf21 insect cells

Specific Activity: ~4000-5000U/mg, where one unit of RSK1 activity is defined as 1nmol phosphate incorporated into 30uM Rsktide per minute at 30C with a final ATP concentration of 100uM.

Quality Control Testing:
Kinase Assay: 0.33.8ng phosphorylates 30uM RSKtide. Assay background was subtracted from the actual counts to yield the results shown below.
MS Tryptic Fingerprint: Confirmed product identity as rat RSK1 with the translated sequence. SDS-PAGE and Coomassie Stain: Purity was assessed by SDS-PAGE and Coomassie blue staining using 3ug of RSK1 active.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Handling Recommendations: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70C.

Molecular Weight:
89.9kD
Source: Recombinant, rat expressed in Sf21 insect cells
Purity: ~95%. Purified using Ni2+/NTA-agarose. Activated with p42 MAPK and PDK1, and repurified by glutathione-, heparin-, and Ni2+/NTA-agarose chromatographies.
Concentration: As reported
Form: Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 300mM sodium chloride, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.