Technical Data
R9417-52B
RSPO3 (R-spondin-3, Protein with TSP Type-1 Repeat, hPWTSR, Roof Plate-specific Spondin-3, hRspo3, Thrombospondin Type-1 Domain-containing Protein 2, PWTSR, THSD2)
Description:
Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled/LRP receptors. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:8000
Western Blot: 0.3-1ug/ml, human skeletal muscle lysates observed on ~28kD bands
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to QHPSAKGNLCPPT, from human RSPO3, at the internal region of the protein (NP_116173.2).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:
Recognizes human RSPO3. Species sequence homology: Bovine, canine, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.